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Core Concepts

Understanding the underlying principles and components of Calibrie helps in building effective and interpretable pipelines.

  • Architecture: How Pipelines, Tasks, and the Context object work together.
  • Linear Compensation: The theory and practice of correcting spectral overlap.
  • Protein Mapping: Standardizing signals from different fluorescent proteins relative to a reference.
  • MEF Calibration: Converting relative units to absolute Molecules of Equivalent Fluorophore using bead standards.
  • Colinearization (Advanced): Addressing non-linear channel responses before linear unmixing.